Comparison of point detection and area detection for point-scanning structured illumination microscopy

Comparison of point detection and area detection for point-scanning structured illumination microscopy

Blog Article

Structured illumination microscopy (SIM) is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging speed.The system resolution is affected by two typical detection modes: Point detection and area detection.However, a systematic analysis of the imaging performance of the different detection modes of the system has rarely been conducted.

In Prescription Dog Food this study, we compared laser point scanning point detection (PS-PD) and point scanning area detection (PS-AD) imaging in nonconfocal microscopy through theoretical analysis and simulated imaging.The results revealed that the imaging resolutions of Daily Dresses PS-PD and PS-AD depend on excitation and emission point spread functions (PSFs), respectively.Especially, we combined the second harmonic generation (SHG) of point detection (P-SHG) and area detection (A-SHG) with SIM to realize a nonlinear SIM-imaging technique that improves the imaging resolution.

Moreover, we analytically and experimentally compared the nonlinear SIM performance of P-SHG with that of A-SHG.